Cardioprotective Effect of Portulaca Oleracea L. (Purslane) against Clozapine-Induced Cardiotoxicity in Rats
Published: 2021-04-20
Page: 101-111
Issue: 2021 - Volume 4 [Issue 1]
Eman Hassan
*
Department of Biochemistry and Nutrition, Faculty of Women for Arts, Science and Education, Ain Shams University, Egypt.
Enas Ali
Department of Biochemistry and Nutrition, Faculty of Women for Arts, Science and Education, Ain Shams University, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Aim: To investigate the protective role of purslane (regla) on clozapine-induced toxicity in rats.
Methods: Fifty male Sprague–Dawley rats were divided into five groups (10 rats per group) as follows: Group1 (control group): Rats injected daily with buffered saline for 21 days. Group2 (cardiotoxic group): Rats injected with clozapine (15mg/kg body weight) for 21 days. Group3: Rats injected daily with clozapine (15mg/kg body weight) for 21 days + 10% of regla water seed extract. Group4: Rats injected daily with clozapine (15mg/kg body weight) for 21 days + 10% of regla dry leaves water extract. Group5: Rats injected daily with clozapine (15mg/kg body weight) for 21 days + 10% of regla juice.
Results: Serum marker enzymes as creatinine kinase, lactate dehydrogenase, AST and troponin T were estimated, as well as inflammatory markers; E-selectin, myeloperoxidase (MPO) and CRP. Caspase-3 and MDA were also estimated. The result showed that purslane seeds, dry leaves extract or juice have ameliorative effect on clozapine-induced cardiac toxicity in rats through decreasing serum CK, LDH, AST and Troponin enzyme activities, serum E-selectin, CRP, myeloperoxidase, caspase-3 and MDA levels compared to cardiotoxic group with purslane juice being the most effective treatment.
Conclusion: It can be concluded that purslane juice, dry water extract or seeds extract can be used for the protection of clozapine induced toxicity.
Keywords: Cardiac toxicity, purslane, clozapine, rats